Annan lost 3-1 against relegated Dumbarton, meaning they will compete in the William Hill League One relegation play-offs.
Ryan Blair scored first for the home team, and Annan's Scott Hooper was shown a red card shortly after the break. In spite of Tommy Goss equalizing, goals from Tony Wallace and James Hilton sealed the victory for Dumbarton.
Cove Rangers secured their spot in the promotion play-offs after a 2-2 draw with Kelty Hearts at Balmoral Stadium. Adam Emslie and Mitch Megginson scored for Rangers, but Kelty Hearts fought back with goals from Craig Johnston and Ross Cunningham.
Queen of South defeated Stenhousemuir 2-0 at Palmerston Park, ensuring their place in the top four.
Inverness beat champions Arbroath 3-0, officially confirming their spot in League One for the next season.
Alloa came from behind to win 2-1 against Montrose with a late goal from Luke Rankin, moving them into fourth place above Stenhousemuir.
Peterhead clinched the League Two title with a 1-0 victory over East Fife at Balmoor Stadium. Peter Pawlett's goal in the 29th minute secured the win and gave them a four-point lead.
Bonnyrigg Rose kept their hopes of avoiding relegation alive with a thrilling 2-1 win over Spartans. Murray Aiken scored first for Rose, Blair Henderson equalized, but Aaron Arnott's stoppage-time goal secured the win.
Elgin, aiming for the play-offs, won 3-0 against Stranraer, while Clyde triumphed 4-1 over Stirling.